Dilution Guide
Dilution arithmetic describes the relationship between an amount of material and a final solution volume. It does not determine a dose, establish solvent compatibility, or make a research material safe for administration.
Mass divided by final volume
Concentration = mass ÷ final volume. For an arithmetic example only, 10 mg in a final solution volume of 2 mL gives 5 mg/mL, or 5,000 micrograms/mL. This assumes complete dissolution and an accurately known amount; it is not a recipe for a particular product.
Diluting an existing solution
For the same solute, C₁ × V₁ = C₂ × V₂, where V₂ is the final total volume. For example, preparing 5 mL at 2 mg/mL from a 10 mg/mL stock requires 1 mL of stock, brought to a final volume of 5 mL with a compatible diluent specified by a validated protocol.
Keep units consistent
1 mg equals 1,000 micrograms; 1 mL equals 1,000 microliters. Use matching units on both sides of an equation. Mass-based arithmetic does not by itself convert to particle counts, biological activity units, or molar concentration.
Arithmetic is not a handling protocol
Solvent selection, final volume measurement, dissolution, adsorption, storage, stability, and contamination control depend on the material and experimental method. Do not apply one generic reconstitution recipe to all catalog products. Use confirmed documentation and your laboratory’s validated procedures.
For research use only. Not for human or veterinary use. Educational content is not medical advice.